When Peter's transformation slash transportation is through, before he's even opened his eyes to see where he is, the first thing he notices is that his body is different. His brain wants to supply him with the word "wrong" but it doesn't, actually, feel wrong. It doesn't feel not his. It just feels, well, different.
Which is a surprising way for it to feel, honestly! If he'd thought about the possibility that he'd instantly shapeshift as soon as he embarked on his adventure he'd have expected it to feel either very weird and wrong and hard to get used to (not likely, his powerset is very friendly) or natural and normal and unspecial. The fact that it doesn't feel normal but it doesn't feel bad either is interesting.
Of course in hindsight it's obvious why that'd be. He's not in his original body, and he doesn't want to act like he is, and he wants to know what changed, be able to notice it. He's feeling glad that this is how it works, after all, which suggests that it's the right way for it to work.
So, in what ways is his body different?
His thighs don't touch. He hadn't had a thigh gap in long enough he no longer even noticed it, so this is noticeable. He's thinner, overall, slimmer and more slender. His elbows are touching his lower ribs, so he has less pronounced back and arm muscles and his shoulders are less broad. He feels lighter, overall. His hair is longer, though, actually very long. It's heavy and in a single thick braid that goes past his waist.
His cock is, in fact, kind of noticeably heavier.
But it's not just his body. His clothes are different, too. He's wearing a crop top and a skirt that goes almost two-thirds of the way down his thighs, then the zettai ryouiki, then the stockings, then flats.
Peter notices all of this very quickly, and when he opens his eyes and reaches for his long braid he knows exactly what he'll see.